Metabolome Facility Bordeaux

Bordeaux Metabolome Facility provides equipments and expertise for the study of metabolome and metabolic fluxes. The facility is mostly dedicated to the study of plant or plant-derived products.

EQUIPMENTS

• A 500 MHz NMR spectrometer (2001, 2010) and its environment including a cryoprobe (NMR Bruker 5 mm 13C optimized, 2010)
• A 600 MHz NMR spectrometer (2008) and its environment including a LC-NMR probe (2008)
• A QqTOF LC-MS system (2007)
• An ion-trap LC-MSⁿ system (2007)
• A GC-MS system (2006)
• A nanoLC-Qtrap-MS system(2008-2009)
• An UHPLC system (2010)
• A robotized system for high-throughput phenotyping with microplates (2009)

SERVICES

METABOLOME, LIPIDOME

• Fingerprints or metabolic profiles with 1H-NMR or LC-MS,
• Quantitative metabolomics : metabolic profiles with quantitative proton NMR or UHPLC,
• Lipidomics: identification and quantification by HPTLC, GC or GC-MS of lipids or fatty acids,
• Structural analysis of phenolics and interactions between biomolecules using multidimensional NMR,
• Biological activities of phenolics.

METABOLISM and FLUXOME

• Analysis of lipid metabolism pathways using labeling and HPTLC,
• Measurement of isotopic enrichments after labeling with stable isotopes (1H and 13C-NMR) for the quantification of metabolic fluxes.

RELATED SERVICES

• Extractions adapted to the chosen biochemical analysis, tissue or matrix,
• Statistical analyses of metabolome data,
• Handling and storage of the produced data and metadata (data bases including MeRy-B) in collaboration with the Bioinformatics Platform,
• Personalized training of students and scientists for metabolomics technologies and tools (analytical and statistical),
• Organization of workshops and scientific meetings.

APPLICATION DOMAINS

The Metabolome Facility supports studies of plant ecophysiology, functional genomics and genetics for regional, national and international research programs. It is also used for the study of plant-derived products. Most studied topics include:

• acquisition and interpretation of metabolomic profiles,
• studies of the dynamics of metabolism,
• functional analyis of lipid metabolism.

TECHNOLOGICAL DEVELOPMENTS

• Robotisation of sample grinding and weighing,
• Obtention of rapid metabolic signatures by high-throughput 1H-NMR,
• Testing, feedback and uploading of the metabolome data base (MeRy-B, collaboration with the Bioinformatics Platform),
• Lipidomics of plant samples,
• Transfer of high-throughput metabolic phenotyping to diverse models,
• Constitution of a library of reference compounds and corresponding spectra,
• Identification of compounds using LC-SPE-NMR and multidimensional NMR.

HIGHLIGHTS

• Participation in 7 European projects in 2010.
• Participation in 9 ANR projets in 2010.
• Implication in the actions of the “French Metabolomics and Fluxomics Network”, including the organization of Scientific Meetings (2005, 2006, 2008, 2010 and 2011) and a Training Workshop in 2009.
• Writing of four « Metabolome » book chapters in 2009-2010.