Plateforme Protéome

“Plateforme Protéome” aims at meeting the needs in proteomics-related research activities of academic and private laboratories and at being an open center for the entire community for the provision of services, materials and expertise. Specifically “Plateforme Protéome” provides services in the field of protein separation by electrophoresis and the identification and / or protein analysis by mass spectrometry. Since February 2012, the "Platform Proteome" is certified ISO 9001:2008 by LRQA. The certified Management Quality System covers our research, technological development and expertise in mass spectrometry and proteomics research in the fields of Biology, Health and Agriculture.

 

Equipments

Mono-dimensional electrophoresis (BioRad)

Two-dimensional electrophoresis : small gels 6 cm (Invitrogen) & large gels 24 cm (Amersham)

GS-800 Calibrated Imaging Densitometer (BioRad)

OffGel fractionator (Agilent 3100)

Pharos FX™ Plus Molecular Imager® system (BioRad)

Image analysis softwares: ImageMaster™ 2D Platinum v5.0 (Amersham Biosciences), Quantity One 4.6.2 (BioRad), PDQuest 8.0.1 (BioRad), Progenesis SameSpots V2.0 (Non-Linear Dynamics)

Spot cutter EXQuest (BioRad)

Mass spectrometers :

Ion Trap LCQ DecaXP+ (ThermoFinnigan) coupled with a nanoLC system (LC Packings)

LTQ Orbitrap XL ETD (Thermo Electron) coupled with a nanoLC system (Dionex)

nanoLC system coupled with a Probot robot (Dionex) for LC-MALDI application

Q-TOF Micro (Waters) coupled with CapLC system

MALDI-TOF-TOF UltraFlex III (Bruker Daltonics) equipped with a High-Mass detector HM2 (CovalX)

MALDI-Q-TOF Premier (Waters)

Mass analysis softwares : Bioworks 3.3.1, DeNovoX 1.0, ClinProt Tools 2.2, BioTools 3.2, WarpLC 1.2, Proteinscape 1.2, Proteome Discoverer 1.3, Progenesis LC-MS 4.0, ProteinLynx GlobalServer, Modiro 1.1.

Search algorithms : Mascot, Sequest, Phenyx.

 

Services

Protein separation: mono-dimensional electrophoresis (SDS-PAGE, BN-PAGE, 16-BAC-PAGE, IEF, Experion), two-dimensional electrophoresis (IEF/SDS-PAGE, BN/SDS-PAGE, 16-BAC/SDS-PAGE)

Isoelectrofocalisation in liquid stream (Rotofor system)

Western blot

Staining of protein gels: blue or colloidal Coomassie, silver, sypro, ruthenium, proQ Diamond

Scanning of gels and image analysis

Protein digestion by endoprotease (Tryspin, LysC, GluC, Chymotrypsin …)

Protein identification in gel or solution by MALDI-TOF or LC-MS/MS mass spectrometry

Search phosphorylation by ETD-MS/MS and/or neutral loss MS³

Enrichment of phosphopeptides

De novo sequencing

Measurement of molecular weight protein

Relative quantification : iTRAQ labelling, SILAC or Label-free

 

Application areas

« Plateforme Protéome » provides support for research in biology, whatever their field of study (genetics, biochemistry, microbiology, plant physiology, neuroscience, oncology, ecology, ...)

 

Technological developments

PIR Technology (Protein Interaction Reporter)

Introduction and technology validation LabelFree

 

Some highlights (national and international actions)

Participation in a European project since 2007.

Participation in 3 ANR in 2010.

Involvement in the animation of Société Française d'Electrophorèse et d'Analyse Protéomique (SFEAP)